MASS SPECTROMETRY-BASED PROTEOMICS
Contact person
Hans Christian Beck, Associate Professor, PhD, Dept. of Clinical Biochemistry, Odense University Hospital
hans.christian.beck@rsyd.dk, https://portal.findresearcher.sdu.dk/da/persons/hcbeck
Research aim
We aim at identification and molecular characterization of novel disease biomarkers in cell cultures, biopsies and body fluids in clinical settings.
We use mass spectrometry-based proteomics technology to study properties and functions of proteins in health and disease.
Specifically, but not exclusively, we focus on the molecular and cellular aspects of complications in arterial wall in diabetes, and the identification biomarkers for cardiovascular diseases such as abdominal aortic aneurysms, acute myocardial infarct, and cardiogenic shock.
Moreover, we aim at devoloping mass spectrometry-based proteomics to be at use in daily clinical diagnostic routine.
Project examples
- Identification and characterization of biomarkers for abdominal aortic aneurysms
- Biomarkers for acute myocardial infarct and cardiogenic shock
- Diagnostic protein biomarkers for asbestos malignant mesothelioma biomarkers
- Proteomics methods for amyloidosis subtyping
- Plasma proteins in twins
Available infrastructures and competences
The proteomics laboratory at OUH is equipped with world-class mass spectrometers, associated bioanalytical, bioinformatics, and bio-statistical platforms.
Collaborators, postdocs and PhD-students
Our group collaborates with clinicians from leading Danish Hospitals and a number of researchers and research groups from Denmark and abroad.
PhD students:
2022-2025: Nicolai Bjødstrup Palstrøm,
University of Southern Denmark/Odense University Hospital
2020 – 2023: Mostafa Ejtehadifar and Sara Zahedi
Computational and Experimental Biology Group,
NOVA MEDICAL SCHOOL / Faculdade de Ciências Médicas, Universidade Nova de Lisboa, PORTUGAL
Ongoing funding
- Danish Heart Foundation
- Odense University Hospital Research Foundation
- Janssen Pharmaceuticals
- AP Møller Foundation (A. P. Møller Fonden)
- Innovation Fund Denmark
Publication examples
- Determining Plasma Protein Variation Parameters as a Prerequisite for Biomarker Studies—A TMT-Based LC-MSMS Proteome Investigation.
Lou-Ann C Andersen, Nicolai Bjødstrup Palstrøm, Axel Diederichsen, Jes Sanddal Lindholt, Lars Melholt Rasmussen, Hans Christian Beck. Proteomes. 2021; 9 (4):47
- STEMI, cardiogenic shock, and mortality in patients admitted for acute angiography: associations and predictions from plasma proteome data.
Debrabant B, Halekoh U, Soerensen M, Møller JE, Hassager C, Frydland M, et al. Shock. 2021;55(1):41-7.
- Comparison of Strategies for Increasing Coverage of Low-Abundant Human Plasma Proteins Palstrøm NB, Rasmussen LM, Beck HC. Affinity Capture Enrichment versus Affinity Depletion: A. International journal of molecular sciences. 2020;21(16):5903.
- Data imputation in merged isobaric labeling-based relative quantification datasets.
Palstrøm NB, Matthiesen R, Beck HC. Data imputation in merged isobaric labeling-based relative quantification datasets. Mass Spectrometry Data Analysis in Proteomics: Humana, New York, NY; 2020. p. 297-308.