Why is 3D better?

Collaborating with CelVivo ApS, we develop novel in vitro liver tissue models based on 3D cell culture spheroids and organoids. Our work focuses on liver fibrosis, fatty liver disease and toxicology models. 

Relevant publications

1. Vej-Nielsen, J. M.; Rogowska-Wrzesinska, A. (2021): 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids. Methods Mol Biol 2273, 159-171. http://dx.doi.org/10.1007/978-1-0716-1246-0_11.
2. Štampar, M.; Sedighi Frandsen, H.; Rogowska-Wrzesinska, A.; Wrzesinski, K.; Filipič, M.; Žegura, B. (2021): Hepatocellular carcinoma (HepG2/C3A) cell-based 3D model for genotoxicity testing of chemicals. Sci Total Environ 755, Pt 2, 143255. http://dx.doi.org/10.1016/j.scitotenv.2020.143255.
3. Frandsen, H. S.; Štampar, M.; Vej-Nielsen, J. M.; Žegura, B.; Rogowska-Wrzesinska, A. (2021): Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics. Methods Mol Biol 2273, 173-188. http://dx.doi.org/10.1007/978-1-0716-1246-0_12.
4. Wojdyla, K.; Wrzesinski, K.; Williamson, J.; Fey, S. J.; Rogowska-Wrzesinska, A. (2016): Acetaminophen-induced S-nitrosylation and S-sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids. Toxicol Res (Camb) 5, 3, 905-920. http://dx.doi.org/10.1039/c5tx00469a.
5. Wrzesinski, K.; Rogowska-Wrzesinska, A.; Kanlaya, R.; Borkowski, K.; Schwämmle, V.; Dai, J.; Joensen, K. E.; Wojdyla, K.; Carvalho, V. B.; Fey, S. J. (2014): The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments. PLoS One 9, 9, e106973. http://dx.doi.org/10.1371/journal.pone.0106973.