NEBcutter
http://nc2.neb.com/NEBcutter2/ This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once.
Reverse Complement
https://www.bioinformatics.org/sms/rev_comp.html Reverse Complement converts a DNA sequence into its reverse, complement, or reverse-complement counterpart.
Blast
https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome A BLAST search allows you to compare a sequence of interest with a database of sequences for homology. It can be used to align different types of sequences.
Primerblast
https://www.ncbi.nlm.nih.gov/tools/primer-blast/ Finding primers specific to your PCR template
Ensembl
https://www.ensembl.org/Homo_sapiens/Info/Index Information about genes, transcripts, sequences, splicing isoforms, exon sequences, gene coordinates etc.
Encode Project
https://www.encodeproject.org/ Encyclopedia of DNA Elements. Experimental data of among other things RNA-protein binding using eCLIP and RNA-seq data
Geuvadis
https://www.internationalgenome.org/data-portal/data-collection/geuvadis This collection contains RNA-seq data generated by the Geuvadis consortium. The data includes mRNA and small RNA sequence data for over 460 samples from the 1000 Genomes project, including samples from five of the 1000 Genomes populations (CEU, FIN, GBR, TSI and YRI)
dbSNP
https://www.ncbi.nlm.nih.gov/snp/ database of SNPs
Fruitfly
https://www.fruitfly.org/seq_tools/splice.html Splice site prediction site